Decreasd autologous fluorescence in flow cytometry of cultured cells.

To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.