Determination of osthole in Fructus Cnidii and Fuyanling Capsule by RP-HPLC

Objective: To establish the determination method for osthole in Cnidii and Fuyan ling Capsule(Fructus Cnidii, Radix Sophorae Flavescentis, Herba Agrimoniae, Radix Stemmonae, etc.). Methods: RP HPLC was used with Alltima C 18 column (250mm×4.6mm,5μm), detection wavelength at 322nm, mobile phrase: methanol water (75∶25), flow rate: 1mL·min -1 . Results: Regression equation of Fuyanling capsules is Y =22957x-816, and r=0.9999 with repetition rate RSD 1.45%. Conclusion: This method is simple, easy and reliable.