Study of cell cycle synchronization in fetal fibroblast by serum starvation

【Objective】The cell cycle of BFF treated by different starvation methods and starvation time was examined. 【Methods】BFF was treated with serum direct starvation or gradient starvation. 【Results】It showed that treatment of BFF with serum direct starvation or gradient starvation resulted in a significant increasing in percentage of cells arrested at G0/G1 stage in comparision with that of control group (P 0.05),but there was no significant difference between the serum direct starvation and gradient starvation groups. When BFF was treated with serum direct starvation for 3 d,the percentage of cells in G0/G1 stage are significantly higher than that of 0,1,2 and 6 d (P 0.05).【Conclusion】BFF treated with serum direct starvation or gradient starvation can efficiently arrest them at G0/ G1 stage,and the optimum duration of serum starvation is 3 d.