All-optical manipulation of retinal circuits in non-human primate explants

Here, we present recent advancements enabling all-optical investigations of cultured non-human primate retinal explants. We have implemented two-photon calcium imaging at a millimeter-scale field-of-view, optimizing the protocols for volumetric acquisition and simultaneous holographic and visual stimulation from a single objective. We discuss the ongoing functional study of primate explants expressing a calcium indicator (GCaMP8m). These developments pave the way toward elucidating the functional architecture of the fovea and advancing our understanding of the fundamental mechanisms underlying human visual processing.