红球菌
拉伤
生物转化
细菌
分解代谢
代谢物
化学
生物降解
微生物学
生物修复
氧化磷酸化
16S核糖体RNA
转化(遗传学)
生物化学
代谢途径
氯霉素
微生物
活性污泥
生物
新陈代谢
基因
酶
抗生素
有机化学
工程类
解剖
废物管理
污水处理
遗传学
作者
Ke Shi,Chenlei Guo,Xiaodan Ma,Bin Liang,Aijie Wang
出处
期刊:Chinese Journal of Biotechnology
日期:2021-10-25
标识
DOI:10.13345/j.cjb.210434
摘要
Microorganisms are the dominant players driving the degradation and transformation of chloramphenicol (CAP) in the environment. However, little bacterial strains are able to efficiently degrade and mineralize CAP, and the CAP degrading pathways mediated by oxidative reactions remain unclear. In this study, a highly efficient CAP-degrading microbial consortium, which mainly consists of Rhodococcus (relative abundance >70%), was obtained through an enrichment process using CAP-contaminated activated sludge as the inoculum. A bacterial strain CAP-2 capable of efficiently degrading CAP was isolated from the consortium and identified as Rhodococcus sp. by 16S rRNA gene analysis. Strain CAP-2 can efficiently degrade CAP under different nutrient conditions. Based on the biotransformation characteristics of the detected metabolite p-nitrobenzoic acid and the reported metabolites p-nitrobenzaldehyde and protocatechuate by strain CAP-2, a new oxidative pathway for the degradation of CAP was proposed. The side chain of CAP was oxidized and broken to generate p-nitrobenzaldehyde, which was further oxidized to p-nitrobenzoic acid. Strain CAP-2 can be used to further study the molecular mechanism of CAP catabolism, and has the potential to be used in in situ bioremediation of CAP-contaminated environment.
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