Kitasatospora hibisci sp. nov., a novel actinomycete with antimicrobial potential isolated from rhizosphere soil of Hibiscus tiliaceus in Guangxi

A novel Kitasatospora strain, designated as HNM1077 T , was isolated from rhizosphere soil of Hibiscus tiliaceus collected from the bank of the Beilun River in Dongxing City, Guangxi Province, China. The novel strain exhibited antimicrobial activity against a wide range of microbes, particularly Gram­-negative bacteria and plant pathogenic fungi. Based on the alignment of 16S rRNA gene sequences, strain HNM1077 T was closely related to Kitasatospora aburaviensis NRRL B-2218 T (99.51%). Comparisons based on genome sequencing showed that strain HNM1077 T was distinguished from its nearest species by average nucleotide identity of 94.36% and a digital DNA–DNA hybridization value of 54.3%. The G+C content of strain HNM1077 T was 73.1 mol%. The main fatty acids of strain HNM1077 T were iso -C 15:0 (21.5%), anteiso -C 15:0 (11.7%), iso -C 16:0 (8.6%), iso -C 17:0 (9.3%) and anteiso -C 17:0 (8.7%). The predominant menaquinone was MK-9 (H 8 ). The major polar lipids of strain HNM1077 T consisted of phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol and glycosylphosphatidylinositol. Based on these polyphasic taxonomic results, strain HNM1077 T represents a novel Kitasatospora species, for which the name Kitasatospora hibisci sp. nov. is proposed. The type strain is HNM1077 T (=CCTCC AA 2024052 T =KCTC 59330 T ).