球形红杆菌
类胡萝卜素
采光综合体
光合作用
生物合成
细菌叶绿素
光防护
光养
绿色体
绿色硫细菌
紫色细菌
生物化学
化学
红杆菌属
生物
光合反应中心
突变体
光系统II
基因
作者
George A. Sutherland,Pu Qian,C. Neil Hunter,David J. K. Swainsbury,Andrew Hitchcock
出处
期刊:Methods in Enzymology
日期:2022-01-01
卷期号:: 137-184
被引量:1
标识
DOI:10.1016/bs.mie.2022.04.001
摘要
Carotenoids are important photosynthetic pigments that play key roles in light harvesting and energy transfer, photoprotection, and in the folding, assembly, and stabilization of light-harvesting pigment-protein complexes. The genetically tractable purple phototrophic bacteria have been useful for investigating the biosynthesis and function of photosynthetic pigments and cofactors, including carotenoids. Here, we give an overview of the roles of carotenoids in photosynthesis and of their biosynthesis, focusing on the extensively studied purple bacterium Rhodobacter sphaeroides as a model organism. We provide detailed procedures for manipulating carotenoid biosynthesis, and for the preparation and analysis of the light-harvesting and photosynthetic reaction center complexes that bind them. Using appropriate examples from the literature, we discuss how such approaches have enhanced our understanding of the biosynthesis of carotenoids and the photosynthesis-related functions of these fascinating molecules.
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