The air-dried flowers of Chrysanthemum parthenium (L.) Bernh. were subjected to hydrodistillation for 3 h using a Clevenger type apparatus. The essential oil was analyzed by GC and GC-MS. Nineteen components were characterized, representing 98.9 % of the total components detected. The oil of the major constituents was identified as camphor (61.1 %), camphene (9.2 %), farnesol (4.6 %), bornyl acetate (3.5 %), chrysanthenon (3.1 %), and borneol (2.9 %). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar diffusion method. Activity was observed against two Gram-positive and one Gram- negative bacteria.