溴化乙锭
化学
激发
激发态
原子物理学
蓝宝石
双光子激发显微术
激光器
光子
分子物理学
DNA
光学
物理
生物化学
量子力学
作者
Henryk Malak,Felix N. Castellano,Ignacy Gryczyński,Joseph R. Lakowicz
标识
DOI:10.1016/s0301-4622(97)00017-3
摘要
We examined the steady state and time-resolved emission of DNA stained with ethidium bromide (EB) when excited with 90 fs pulses from a mode-locked titanium sapphire laser. Over the wavelength range from 840 to 880 nm EB-DNA was found to display two-photon excitation, with a cross-section near 7 x 10(-50) cm4s/photon. Frequency-domain intensity decay measurements revealed similar multi-exponential intensity decays for one- and two-photon excitation. Time-resolved anisotropy decay measurements revealed similar correlation times, but different amplitudes as has been observed previously for two- versus one-photon excitation. These results indicate that two-photon excitation of EB-DNA can be accomplished with the fundamental output of a Ti:sapphire laser without obvious heating or perturbation of the DNA.
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