We are developing a laboratory‐scale protein structure analysis beamline based on our portable synchrotron MIRRORCLE‐20SX, which is a 20 MeV machine. Using the crystal as a target in the central orbit of the synchrotron, we will obtain parametric X‐ray, the spatially resolved monochromatic X‐ray. Our final goal is a development of protein crystallography basis cooperated with the protein group of the Nagahama Institute of Bio‐science and Technology, which performs screening, crystallization, and protein structure analysis. The METI Kansai funded this program.