增殖细胞核抗原
软骨
阿格里坎
超微结构
免疫组织化学
化学
髁突
原位杂交
解剖
病理
细胞生物学
生物
信使核糖核酸
骨关节炎
医学
关节软骨
替代医学
基因
生物化学
作者
Zhiyuan Gu,Ying Hu,Yin-kai Zhang,Jianying Feng,Jian Hu,Zheng-Kang Zhang
出处
期刊:PubMed
日期:2005-02-01
卷期号:14 (1): 33-6
被引量:2
摘要
To study the tissue layers and their function of the cartilage in mandibular condyle in rabbits.Six adult Japanese white rabbits were subjected. Their temporomandibular joints were studied by immunohistochemistry for FGFR3 and PCNA, and in situ hybridization for aggrecan and collagen II mRNA expression, as well as ultrastructure.The upper proliferative cells did not express FGFR3, but the lower proliferative cells expressed FGFR3. Only few cells in the upper proliferative layer were PCNA positive, but all cells in the lower proliferative layer were positive for PCNA. No collagen II mRNA expression was found in the upper proliferative cell, but aggrecan and collagen II mRNA coexpressed in the lower proliferative layer. The cells in both layers were different in ultrastructure.The cartilage in mandibular condyle should have the 5 following tissue layer: fibrous layer, proliferative layer, transitional layer, cartilaginous layer and calcified cartilaginous layer. The cells in the proliferative layer are undifferentiated and the cells in the differentiated layer are prechondrocytes.
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