Determination of Elimination Ratio of Hydroxyl Radicals in Traditional Chinese Medicine by HPLC

OBJECTIVE To establish a HPLC method for the determination of elimination ratio of hydroxyl radicals by Traditional Chinese Medicine.METHODS Benizoic acid reacted with hydroxyl radicals produced by Co2+ and H2O2,and main hydroxylated products were made.Extractives of Traditional Chinese Medicine eliminated hydroxyl radicals in solution and reduced the amounts of the products.Then the elimination ratio of hydroxyl radicals by Traditional Chinese Medicine was determined indirectly.RESULTS The optimal determination condition was found out and the reaction system of benizoic acid and hydroxyl radicals was analyzed.CONCLUSION The method can be applied to screen the hydroxyl radical scavenger and study the hydroxyl radical.