光电二极管
化学
荧光
检出限
分析化学(期刊)
色谱法
光电子学
光学
材料科学
物理
作者
Kazuhiro Morioka,Moeko Osashima,Norihiro Azuma,Kuizhi Qu,Akihide Hemmi,Atsushi Shoji,Hiroya Murakami,Norio Teshima,Tomonari Umemura,Katsumi Uchiyama,Hirochika Nakajima
出处
期刊:Talanta
[Elsevier]
日期:2022-02-01
卷期号:238: 122994-122994
被引量:5
标识
DOI:10.1016/j.talanta.2021.122994
摘要
We developed a small fluorescence microplate reader with an organic photodiode (OPD) array. The OPD array has nine OPDs that have a large light receiving area (9.62 mm2 per one OPD). Since the OPD array is fabricated on a flat glass plate, it can be placed just below microwells and can detect fluorescence emitted through the entire surface of the microwell bottom. The analytical performance of the developed plate reader was evaluated by measuring an aqueous solution of resorufin. The limit of detection (LOD) for resorufin (0.01-0.05 μM) was lower than that obtained with a plate reader equipped with nine inorganic photodiodes developed in a previous study (0.30 μM) and a commercially available microplate reader (0.16 μM). These results indicate that the large light receiving area improves the detection performance of the system. In addition, the developed reader was successfully used to quantify immunoglobulin A (IgA) in human saliva. The LOD for IgA was estimated to be 1.2 ng/mL, which is low enough to objectively evaluate human stress.
科研通智能强力驱动
Strongly Powered by AbleSci AI