细粒棘球绦虫
巨噬细胞
生物
糖酵解
丙酮酸激酶
巨噬细胞极化
己糖激酶
表型
细胞因子
M2巨噬细胞
缺氧诱导因子
体外
癌症研究
细胞生物学
免疫学
内分泌学
生物化学
基因
新陈代谢
动物
作者
Yuehan Feng,Rui‐Hua Xu,Jing Lü,Jun Hou,Lianghai Wang,Di Dong,Xian Wang,Xiaofang Wang,Xiangwei Wu,Xueling Chen
出处
期刊:Journal of Helminthology
[Cambridge University Press]
日期:2023-01-01
卷期号:97
标识
DOI:10.1017/s0022149x23000548
摘要
Human cystic echinococcosis (CE) is a zoonotic disorder triggered by the larval stage of Echinococcus granulosus (E. granulosus) and predominantly occurred in the liver and lungs. The M2 macrophage level is considerably elevated among the liver of patients with hepatic CE and performs an integral function in liver fibrosis. However, the mechanism of CE inducing polarisation of macrophage to an M2 phenotype is unknown. In this study, macrophage was treated with E. granulosus cyst fluid (EgCF) to explore the mechanism of macrophage polarisation. Consequently, the expression of the M2 macrophage and production of anti-inflammatory cytokines increased after 48 h treatment by EgCF. In addition, EgCF promoted polarisation of macrophage to an M2 phenotype by inhibiting the expression of transcriptional factor hypoxia-inducible factor 1-alpha (HIF-1α), which increased the expression of glycolysis-associated genes, including hexokinase 2 (HK2) and pyruvate kinase 2 (PKM2). The HIF-1α agonist ML228 also inhibited the induction of macrophage to an M2 phenotype by EgCF in vitro. Our findings indicate that E. granulosus inhibits glycolysis by suppressing the expression of HIF-1α.
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