Insight into the antibacterial activity and mechanism of allicin and antioxidant of bamboo leaves against Shewanella putrefaciens

Summary The antibacterial activity and mechanism of allicin (All) and antioxidant of bamboo leaves (AOB) against Shewanella putrefaciens was studied. The minimum inhibitory concentration (MIC) and growth curve were used to evaluate the antibacterial activity of combining All and AOB against S. putrefaciens . The inactivation mechanism was reflected by the changes in Na + /K + ‐ATPase activity, confocal laser scanning microscopy (CLSM), and the leakage of nucleic acid and protein. Scanning electron microscopy (SEM) was used to observe the morphology of bacterial cells and crystal violet biofilm reflected the ability to inhibit the formation of biofilm. Results showed that after being treated by All and AOB, S. putrefaciens was effectively inactivated, and the combined treatment had the strongest inactivation effect. The cell wall and cell membrane were destroyed by All and AOB, resulting in the release of cell contents. The results of Na + /K + ‐ATPase activity demonstrated that the membrane permeability could be changed, and the cell structural proteins could be destroyed by All and AOB. SEM observation further proved the effects of All and AOB on bacterial morphology destructing. Therefore, the combination of All and AOB can be used as an efficient method for the preservation of aquatic products.