吖啶橙
串联
化学
荧光
酶
酶分析
生物化学
芳香族L-氨基酸脱羧酶
分子生物学
生物
细胞凋亡
材料科学
物理
量子力学
复合材料
作者
Siying Ren,Wen‐Chao Geng,Xuexian Cui,Bian Wu,Zhe Zheng
标识
DOI:10.1002/slct.202203646
摘要
Abstract L–DOPA decarboxylase (DDC) deficiency causes serious progressive neurodegenerative disorders. Developing a sensitive enzyme activity monitoring and inhibitors or activators screening approach is highly on demand for DDC deficiency diagnosis. Here, we report a simple and sensitive fluorescence supramolecular tandem assay based on reporter pair of cucurbit[7]uril and acridine orange for DDC activity monitoring continuously. Furthermore, this strategy demonstrates the potential of STA to screen inhibitors of DDC.
科研通智能强力驱动
Strongly Powered by AbleSci AI