Rapid and global detection and characterization of the constituents in ShengMai San by ultra‐performance liquid chromatography–high‐definition mass spectrometry

Abstract An ultra‐performance liquid chromatography–high definition mass spectrometry (UPLC‐HDMS) method was developed for detection and characterization of the chemical constituents in ShengMai San (SMS), a traditional Chinese medical formula (TCMF). The full‐scan LC‐MS/MS data sets combined with extra mass were acquired within 14 min using UPLC‐HDMS in the MS E mode in a single injection. As a result, 92 compounds were identified by comparing the accurate mass and fragments information with that of the authentic standards as well as by MS analysis and the correlative references data. These constituents included ginsenosides, lignans, steroidal saponins and homoisoflavanones. Among them, 25‐hydroxyginsenosides were discovered in SMS for the first time. Compare with the previous studies, our research detected more compounds and presented more rapid by applying UPLC‐HDMS. It is concluded that a rapid and effective method has been established based on UPLC‐HDMS with the utilization of MS E , which shows high sensitivity and resolution that is suitable for identifying the constituents of SMS, and this method could be applied to other TCMF.