The emergence of nanotechnology is opening new horizons for the application of nanoparticles in analytical chemistry.Nanoparticles are of considerable interest owing to their unique physical and chemical properties and offer excellent prospects for biosensor,becanse of their parge specific surface area and high surface activity.The paper presents here the application of gold nanoparticle and magnetic nanoparticle in the biosensor,and the prospect of the application is also proposed.