Effect of IGF-II and EGF on the Development of ICR Mouse Embryos in Vitro

Objective: To investigate the effection of IGF-Ⅱ and EGF on the development of ICR mouse preimplantation embryos in the CZB culture medium with glucose and insulin in vitro.Methods: ①Experiment1: One-cell ICR mouse embryos were cultured in the CZB medium with glucose and insulin supplemented with IGF-Ⅱ(0,0.1,1,10 and 100 μg/L),and a separate control group of CZB medium was established.②Experiment 2: One-cell ICR mouse embryos were cultured in the CZB medium with glucose,insulin and IGF-Ⅱ(0.1 μg/L) supplemented with EGF(0,0.1,1,10 and 100 μg/L),and a separate control group of CZB medium was established.Blastocyst rates,hatched blastocyst rates and the cell numbers of blastocysts were counted.Results: ①Experiment1: IGF-Ⅱ(0 μg/L) increased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with control group(P0.05).IGF-Ⅱat a dose of 100 μg/L decreased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with the other groups(P0.05).IGF-Ⅱ at a dose of 0.1 μg/L greatly increased the total cell numbers of blastocyst(P0.05).②Experiment 2: EGF(0 μg/L) increased the rate of blastocyst,hatched blastocyst and the total cell numbers of blastocyst compared with control group(P0.05).EGF at doses of 0.1,1 and 10 μg/L increased the rate of blastocyst and hatched blastocyst compared with EGF at doses of 0 and 100 μg/L(P0.05),and EGF at a dose of 0.1 μg/L had the highest rate of hatched blastocyst(P0.05).EGF at a dose of 0.1 μg/L increased the total cell numbers of blastocyst compared with EGF at a dose of 0 μg/L(P0.05).EGF at a dose of 100 μg/L had the lowest rate of the total cell numbers of blastocyst(P0.05).Conclusions: ①IGF-Ⅱ(1,10 μg/L),glucose and insulin showed no significant effect of suppression or promotion among.②EGF(1,10 μg/L) in CZB medium with glucose,insulin and IGF-Ⅱcould involves in the regulationof differentiation of embryonic cells but no proliferation.③IGF-Ⅱ and EGF at a dose of 0.1 μg/L is best for cell culture concentration.④IGF-Ⅱ and EGF at a dose of 100 μg/L have inhibition effect on embryonic cells.