Effect of Daidzein and Genistein on Proliferation and Antioxidation of Mammary Epithelial Cell of Dairy Cow in vitro

Effect of Daidzein and Genistein on the proliferation and antioxidation of mammary epithelial cell of dairy cow were determined in vitro.The experiments were assigned into 9 groups(blank,1,10,100,1 000 ng/mL Daidzein and Genistein).According to MMT,the proliferation experiment results showed that 10,100 ng/mL Genistein and 100,1 000 ng/mL Daidzein significantly improved dairy cow mammary epithelial cell proliferation(P0.05),but all weaker than 10 ng/mL E2 group(P0.05) after 72 h cultured.In the other experiment,the mammary epithelial cell during the logarithm growing period were collected after 24 h cultured to measured the T-SOD,GSH-PX activity,MDA,NO content.Results showed that 100,1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the T-SOD activites and decreased MDA concentration(P0.05).100,1 000 ng/mL Daidzein and 10,100,1 000 ng/mL Genistein significantly increased NO concentration(P0.05).1 000 ng/mL Daidzein and 100,1 000 ng/mL Genistein significantly increased the GSH-PX activites(P0.05).It was proved that proper levels of Daidzein and Genistein can advance the proliferation and antioxidation of mammary epithelial cell of dairy cow.