The apoplastic barriers of the Cynodon dactylon and Paspalum distichum roots are consisted of two layers at young development stage. The inner layer is the endodermis with Casparian walls, suberin lamellae and lignified secondary cell walls. The outer is the exodermis with Casparian walls, suberin lamellae and lignified. The apoplastic barriers of the matured roots of the two species also had two layers with the inner endodermis of Casparian walls, suberin lamellae, lignified secondary cell walls, the suberized and lignified cortex cell walls adjacent to endodermis and the outer exodermis of Casparian walls, suberin lamellae and lignified. The difference is the epidermis cell of the Paspalum distichum having diffused suberin in young roots and the endodermis is lignified very later. The suberized and lignified cortex cell walls adjacent to endodermis of the Cynodon dactylon is lignified very early.