Fusarium oxysporum pernciosium was gotten through tissue separation and the biological characteristics was studied by common methods.The results showed that F.oxysporum could grow on PDA,PSA as well as on the medium(ADA) containing the roots.F.oxysporum have the similar growth on PDA and PSA,but weaker on ADA.The growth temperature of the F.oxysporum was 5~32℃,and the optimum temperature range was 23~29℃.The germination rate of the spore was the highest in the glucose solution and was lower in the root extract solution.The pH value for culturing the myceliun limits from 3~12 and neutral-alkali environment condition was more suitable for its growth.