Pectin mediated synthesis of nano hydroxyapatite-decorated poly(lactic acid) honeycomb membranes for tissue engineering

The fabrication of porous films with bioactive nanoparticles has been arousing interest in nanobiotechnology. The biocompatible nanocomposite membrane could mimic the functions of basement membrane besides the augmented cell functions including adhesion, spreading, proliferation, and differentiation. We have reported the green template synthesis of nano hydroxyapatite (nHAp) using pectin from bitter gourd fruits followed by the fabrication of nHAp (2 wt%, 4 wt%, and 6 wt%) incorporated honeycomb-like poly(lactic acid) (PLA) films to evaluate the effect of nHAp on the surface patterning of PLA films. The use of naturally available bitter gourd with pectin content could efficiently stabilize or envelop the nanoparticles after nucleation process, resulting in reduced particle size. The cell viability over normal cells and cancer cells were evaluated for H-PLA/nHAp films. The present work recommends the biofriendly surface engineering of PLA films with the assistance of nHAp particles for bone tissue engineering.