结合
碳二亚胺
纳米颗粒
超顺磁性
材料科学
内化
生物物理学
磁性纳米粒子
荧光
荧光显微镜
共轭体系
二价
氧化铁纳米粒子
磁场
纳米技术
核磁共振
细胞
化学
生物化学
磁化
聚合物
高分子化学
生物
数学分析
物理
数学
量子力学
冶金
复合材料
作者
Chia-Haw Lee,Chao-Bin Chen,Ting-Hao Chung,Yu‐Sheng Lin,Wen‐Chien Lee
标识
DOI:10.1166/jnn.2010.3019
摘要
A method for fast delivery of proteins conjugated to superparamagnetic iron oxide nanoparticles (SPION) into mammalian cells by applying a strong magnetic field in pulses was proposed. Firstly, SPION were prepared from an alkaline solution of divalent and trivalent iron ions and covalently bound with protein through the activation of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC). After fluorescently labelling, the protein-nanoparticle conjugate was mixed with mammalian cell line and exposed to a pulsed magnetic field for short durations of few milliseconds. Results suggested that superparamagnetic nanoparticles were able to carry proteins into living cells immediately. Cellular internalization of the fluorescently labelled protein-nanoparticle conjugate was proved by the observation of cell fluorescence in a fluorescent microscopy, as well as cell analysis by a flow cytometer. We found that the cellular uptake was accomplished dominantly by the process of bombardment of magnetic nanoparticles.
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