检出限
石墨烯
材料科学
纳米复合材料
透射电子显微镜
扫描电子显微镜
比表面积
化学工程
介孔材料
线性范围
电化学
纳米颗粒
纳米技术
电极
化学
色谱法
催化作用
有机化学
复合材料
物理化学
工程类
作者
Xinshui Ren,Fangfang Wu,Mengdie Wu,Hongmin Gao,Chuanbin Wu,Wendi Mu,Simin Liu,Lawrence Que,Hehua Zhang,Michael Z. Miao,Chaofang Dong,Hongzhi Pan
标识
DOI:10.1016/j.bioelechem.2023.108613
摘要
Cancer antigen 125 (CA125)1 is the most important biological screening indicator used to monitor epithelial ovarian cancers, and it plays a vital role in distinguishing ovarian cancers from benign diseases. Biosensors show great potential in the analysis and detection of disease markers. In this study, we designed electrochemical sensors based on three-dimensional amino-functionalized reduced graphene oxide (3D-rGOF-NH2),2 MgAl layered double hydroxide nanocomposites containing ordered mesoporous carbon (CMK-3),3 and ferrocene carboxylic acids(Fc-COOH)4for the detection of CA125. 3D-rGOF-NH2 possesses good conductivity, a large surface area, and high porosity, enabling more immobilized nanoparticles to be deposited on its surface with excellent stability. CMK-3@Fc@MgAl-LDH nanocomposite was used as a carrier to enhance the immobilization of antibodies and the loading of Fc, conductors to enhance conductivity, and enhancers to gradually amplify the signal of Fc. The surface morphology, elemental composition, and surface groups of the materials were characterized using scanning electron microscopy (SEM),5 transmission electron microscopy (TEM),6 and X-ray diffraction (XRD)7 techniques. The response signal of the electrochemical sensor was measured by DPV. Under the optimal conditions, the electrochemical sensor obtained a linear detection range of 0.01 U/mL-100 U/mL with a detection limit of 0.00417 U/mL.
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