This study aims to assess the antibacterial activity of boric acid ester derived from boron mines, which is utilised as an active component in the paint industry. Multiple product variations were obtained by adding boric acid ester to the base, referred to as the reference sample, known as silk matte paint, and used as an interior paint with active substance concentrations ranging from 0.005% to 0.6%. Escherichia coli (ATCC10536) bacteria were inoculated at 78000 cfu cm−2 into samples with 0.005- 0.075% active substance, and at 24000 cfu cm−2 into samples containing 0.15- 0.6% of the active substance, and the reduction rates were determined by a mathematical equation via multivariate linear regression analysis, relating Escherichia coli reduction percentages, surface energy values, and pH measurements, including the power percentages and gloss values for the reference sample. The highest level of antibacterial effect was observed in the sample containing 0.075% active substance.