Effect of diammonium glycyrrhizinate on peritoneal fibrosis in rats receiving peritoneal dialysis

Objective To investigate the effect of diammonium glycyrrhizinate on peritoneal fibrosis in rats receiving peritoneal dialysis.Methods The male SD rats (clean grade) were randomly divided into 5 group,the rats in normal control group were not given any intervention;the rats in NS group were given 0.9% sodium chloride injection 20ml by intraperitoneal injection daily;the rats in model group were given 4.25% peritoneal dialysis solution 20ml daily,meanwhile,were given lipopolysaccharide (LPS) 0.6mg/kg,3 times a week by intraperitoneal injection;the rats in pentoxifylline treatment group,on the basis of model group,were given pentoxifylline 6mg/kg daily by intraperitoneal injection;the rats in diammonium glycyrrhizinate treatment group,on the basis of model group,were given diammonium glycyrrhizinate 10mg/kg daily by intraperitoneal injection.The trial was performed for continuous 28 days,at the 30th day,2h peritoneal equilibration test was adopted to determine the rat's peritoneum function,and the partial peritoneum tissues of rats were collected and the changes of pathological morphous were observed under light microscope.The experimental data were analyzed with SPSS 13.0.Results There were no significant changes in peritoneum function and structure in normal control group and NS group,however,which in model group were severely damaged (P0.05),and the injury degrees of peritoneum function and structure in diammonium glycyrrhizinate treatment group and pentoxifylline treatment group were comparatively slighter (P0.05),and there were no significant differences in the protective effects on peritoneum between the two groups (P0.05).Conclusion The 4.25% peritoneal dialysis solution can cause peritoneal fibrosis in rats,however,diammonium glycyrrhizinate can improve peritoneal fibrosis and relieve the aggravation of peritoneum structure and function.