On proliferation of white spot syndrome virus(WSSV) within Green Mud Crab(Scylla paramamosain) by real time PCR

Diseases of Scylla paramamosain,a main culture species in sea waters of China are increasing with the growing of culture areas and density.There are still no studies on the susceptibility of S.paramamosain to WSSV and how WSSV proliferates within mud crab.The investigation showed that there were about 8.47% of green mud crabs carried WSSV in natural sea waters in Hainan Province.Subsequently,studies were conducted on green mud crab by oral administration and muscle injection with WSSV.Results demonstrated that green mud crab could be infected with WSSV by oral administration with diseased shrimp,and WSSV could proliferate very quickly within green mud crab.With the number of WSSV increasing,the mortality of crab was on the increase.Results suggested that S.paramamosain was a natural host of WSSV,which could be infected with WSSV by consuming diseased shrimp and become a medium of WSSV in a polyculture system of shrimp and crab.