The LMS (Least-Mean-Square) algorithm, which can obtain the complex transfer function in real-time, allows the changes of noise characteristics and the environment of system, thereby maintaining the convergence performance. Therefore, many algorithms based on the LMS algorithm have been modified to solve for some practical considerations, and the FXLMS (Filtered-X LMS) algorithm has been widely investigated and applied as a feed-forward control scheme for an active control system. In 3-dimensional space system, measurement of the signal (reference and error) is more important than control algorithm. However, almost studies were focused on ANC (Active Noise Control) algorithm. In this paper, FXLMS algorithm is applied to the experiment on the ANC of the 3-dimensional enclosure system. And evaluate the ANC performance by changing the location of reference signal.