209.6: Long-term Functional Survival of Human Stem Cell-derived Islets Microencapsulated in Alginate With CXCL12 in Non-human Primates Without Immunosuppression

Purpose: Human pluripotent stem cell-derived islet-like cells (SC-ILCs) represent a sustainable source for beta-cell replacement to achieve a functional cure for type 1 diabetes. Long-term glycemic correction by SC-ILCs encapsulated in various biomaterials to avoid systemic immunosuppression has only been demonstrated in rodent models. Translation of these approaches in large animals and humans is challenging, due to the robust foreign body and allo- or xeno-immune responses that lead to early graft failure. We reported that SC-islets microencapsulated with alginate that incorporates CXCL12 restored long-term glycemic correction without systemic immunosuppression in mice. Here, we report long-term functional survival of SC-ILCs alginate-microencapsulated with CXCL12 in a non-diabetic (ND) and diabetic (DM) nonhuman primates (NHPs). Methods: Microencapsulated SC-ILCs were transplanted into the greater omental sac of a ND and two DM NHPs. Microcapsules were also transplanted intraperitoneally in parallel in immunocompetent C57BL/6 diabetic mice. Biochemical, immunologic, and hematologic parameters, blood glucose levels, serum C-peptide and intravenous glucose tolerance tests were carried out over 24 weeks. Biopsies of microcapsules from the ND NHP were recovered at 4, 12 and at 24 weeks post-transplantation to assess their survival, function and the local immune responses to the microcapsules. Results: Blood glucose levels of the ND NHP were within normal range throughout the study while insulin requirements of the DM NHPs were consistently reduced post-transplantation onwards allowing exogenous insulin dosing to be reduced by up to 62% relative to pre-transplant requirements. Normoglycemia was restored in transplanted diabetic mice. Serum C-peptide levels of the ND NHP were stable (~200 pM to 700 pM) and random and intravenous glucose-induced C-peptide was detectable in the DM NHPs post-transplant. Recovered SC-ILCs from ND NHP at 1, 12 and 24-weeks as well as those retrieved from the DM NHPs at completion of the study were glucose-responsive. Retrieved microcapsules at all time points in all animals were predominantly free-floating without evidence of fibrotic overgrowth. Messenger RNA transcript and protein expression of islet beta-cell markers were detectable, at 24 weeks post-transplant in the ND NHP, albeit at decreased levels compared to pre-transplantation, by qPCR and immunohistochemistry, respectively. There was no evidence of islet regeneration in the recipient pancreas or significant anti-human (SC-ILC) T-cell mediated immune responses. Conclusions: These preliminary findings support ongoing studies in additional diabetic NHPs transplanted with SC-islets microencapsulated in alginate containing CXCL12 without systemic immunosuppression.