Antimicrobial sponge prepared by hydrophobically modified chitosan for bacteria removal

Hydrophobically modified chitosan (HMCS) prepared by reacting chitosan with dodecyl aldehyde can generate very stable foam when dissolved in mild acidic condition under vigorous mechanical stirring. A durable and lightweight (density of 32 mg/ml) sponge was obtained by freeze-drying the stably formed HMCS foam. In addition to the cationic nature of chitosan, the grafted C12 alkyl chains were also able to help HMCS sponge for capturing E. coli cells (∼4.0 × 108 cells/mg sponge) by intercalating into the outer membrane of E. coli cells. E. coli cells captured on HMCS sponge were found to be mostly dead and easily released into the bulk solution so that the active surface could be continuously regenerated for capturing and killing the rest of alive cells. In comparison with its counterpart (chitosan sponge), HMCS sponge maintained a higher operational stability for the removal of E. coli cells. After 5 repeated cells removal operation, the removal capacity of HMCS sponge could be regenerated back to >90% by thorough washing with ethanol.