细胞毒性
神经母细胞瘤
生物碱
细胞培养
胶质瘤
药理学
去氢骆驼蓬碱
细胞生长
活力测定
生物
长春新碱
传统医学
细胞
化学
生物化学
立体化学
体外
癌症研究
医学
环磷酰胺
化疗
遗传学
作者
Euridice Prado,Marcela Gimenez,Rodríguez Vázquez,José L. Espartero,M.T. Sáenz Rodríguez
出处
期刊:Phytomedicine
[Elsevier]
日期:2007-04-01
卷期号:14 (4): 280-284
被引量:117
标识
DOI:10.1016/j.phymed.2006.12.023
摘要
Uncaria tomentosa inner bark extract is a popular plant remedy used in folk medicine to treat tumor and inflammatory processes. In this study, the anti-tumoral effects of its pentacyclic alkaloid mitraphylline were investigated. Furthermore, its growth-inhibitory and cytotoxic effects on glioma GAMG and neuroblastoma SKN-BE(2) cell lines were studied using cyclophosphamide and vincristine as controls. A colter counter was used to determine viable cell numbers, followed by application of the tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)2-(4-sulfophenyl)-2H-tetrazolium], inner salt, colorimetric method to evaluate cell viability in this cytotoxicity assay. Micromolar concentrations of mitraphylline (from 5 to 40 microM) inhibited the growth of both cell lines. It inhibited the growth of the two cell lines studied in a dose-dependent manner. The IC(50) values were 12.3 microM (30h) for SKN-BE(2) and 20 microM (48 h) for GAMG, respectively. This action suggests that mitraphylline is a new and promising agent in the treatment of human neuroblastoma and glioma.
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