消化(炼金术)
化学
抗氧化剂
阿布茨
超滤(肾)
水解物
肽
食品科学
生物化学
体外
餐食
高效液相色谱法
大小排阻色谱法
色谱法
DPPH
酶
水解
作者
Li Feng,Fei Peng,Xiaojing Wang,Mei Li,Hongjie Lei,Huaide Xu
标识
DOI:10.1016/j.foodres.2018.08.068
摘要
The aim of this study was to isolate and identify antioxidant peptides from defatted walnut meal proteins hydrolysates (DWMPH) prepared by simulated gastrointestinal digestion, and to evaluate the protective effect of the selected antioxidant peptides against oxidative damage to SH-SY5Y cells. The DWMPH were purified by ultrafiltration, gel filtration chromatography and RP-HPLC successively, and finally six major peptides were identified as VRN, NPAN, AHSVGP, SSE, TY and SGGY by UPLC-QTOF-MS. TY and SGGY displayed stronger ABTS radical scavenging activity and higher ORAC value in comparison with other four peptides. Furthermore, 0.5 mg/mL SGGY exhibited significantly protective effect on SH-SY5Y cells against oxidative damage (p < .01). Therefore, it was suggested that walnut meal protein could serve as a potential source of antioxidative peptides through gastrointestinal digestion.
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