磷酸戊糖途径
代谢物
新陈代谢
细胞代谢
代谢组
代谢途径
荧光
生物化学
细胞生物学
生物物理学
生物
作者
Zhuo Zhang,Xiawei Cheng,Yuzheng Zhao,Yi Yang
标识
DOI:10.1146/annurev-anchem-091619-091306
摘要
As the core component of cell metabolism, central carbon metabolism, consisting of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle converts nutrients into metabolic precursors for biomass and energy to sustain the life of virtually all extant species. The metabolite levels or distributions in central carbon metabolism often change dynamically with cell fates, development, and disease progression. However, traditional biochemical methods require cell lysis, making it challenging to obtain spatiotemporal information about metabolites in living cells and in vivo. Genetically encoded fluorescent sensors allow the rapid, sensitive, specific, and real-time readout of metabolite dynamics in living organisms, thereby offering the potential to fill the gap in current techniques. In this review, we introduce recent progress made in the development of genetically encoded fluorescent sensors for central carbon metabolism and discuss their advantages, disadvantages, and applications. Moreover, several future directions of metabolite sensors are also proposed.
科研通智能强力驱动
Strongly Powered by AbleSci AI