去细胞化
心肌梗塞
心脏病学
细胞外基质
医学
心肌纤维化
内科学
血管生成
脂肪组织
纤维化
心功能曲线
生物医学工程
组织工程
心力衰竭
细胞生物学
生物
作者
Liang Qiao,Yan Kong,Yingshen Shi,Aijun Sun,Ruijuan Ji,Chao Huang,Yuquan Li,Xiangqun Yang
出处
期刊:Life Sciences
[Elsevier]
日期:2019-12-01
卷期号:239: 116891-116891
被引量:18
标识
DOI:10.1016/j.lfs.2019.116891
摘要
The aims of the study are to investigate whether the combination of adipose-derived stem cells (ADSCs) with decellularized extracellular matrix (dECM) of myocardium can exert synergistic therapeutic effects on acute MI and the underlying mechanism.Myocardial dECM from fresh porcine myocardium was prepared in an injectable gel, ADSCs were seeded into the myocardial dECM gels, and then the mixture was injected into the myocardium of the infarct border zone after acute MI, which was induced by ligating the left anterior descending coronary artery in male SD rats, to assess the therapeutic potential. The degree of fibrosis was detected by Masson's trichrome. The evaluation of cardiac function was performed by Electrocardiography.Myocardial dECM (2.0%) had a suitable aperture and arrangement for cell growth, and also exhibited suitable biomechanical properties. Four-weeks after treatment in vivo, the combination of ADSCs and myocardial dECM could obviously increase angiogenesis, reduce the degree of fibrosis, and decrease infarct size. Furthermore, the combination treatment exerted significant functional improvement. Compared with ADSCs or dECM group alone, the left ventricular ejection fraction (LVEF) in the combination group was 13.4% and 21.8% elevated, respectively.The combination of ADSCs and myocardial dECM has synergistic effects on cardiac repair in acute MI.
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