The extraction of tilapia skin collagen by using hot water

Objective To explore the methods for r extracting collagen from tilapia skin and the components of amino acid of collagen. Methods The tilapia skin collagen was extracted by using hot water. Results The optimum conditions were the extraction temperature of 90℃, the pH value of 6.0, liquid/solid ratio of 1:13 and extraction time of 2h. The highest extraction rate of collagen protein from Tilapia skin was 265.3mg per gram raw material. The rate of oxyproline hydroxyproline (the typical amino acid of collagen protein) reached 9.7%. Conclusion The mothod is easy and feasible.