突变体
振荡(细胞信号)
抄写(语言学)
基因
细菌
基因表达
紫胶操纵子
生物
正面反馈
绿色荧光蛋白
生物物理学
化学
分子生物学
遗传学
哲学
工程类
电气工程
语言学
作者
Shihui Shen,Yushu Ma,Yuhong Ren,Dongzhi Wei
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2016-01-01
被引量:1
标识
DOI:10.4014/jmb.1507.07019
摘要
Synthetic oscillators are gene circuits in which the protein expression will change over time. The delay of transcription, translation, and protein folding is used to form this kind of behavior. Here, we tried to design a synthetic oscillator by a negative feedback combined with a positive feedback. With the mutant promoter PLacC repressed by LacIq and PLux activated by AHL-bound LuxR, two gene circuits, Os-LAA and Os-ASV, were constructed and introduced into LacI-deleted E. coli DH5α cells. When glucose was used as the carbon source, a low level of fluorescence was detected in the culture, and the bacteria with Os-ASV showed no oscillation, whereas a small portion of those carrying Os-LAA demonstrated oscillation behavior with a period of about 68.3 ± 20 min. When glycerol was used as the carbon source, bacteria with Os-ASV demonstrated high fluorescence value and oscillation behavior with the period of about 121 ± 21 min.
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