The chemical constituents which have anti-tumor activity from Duchesnea indica were studied by modern chromatography separate technics.Four compounds were isolated by column chromatography and HPLC.By spectroscopic method,their structures were identified as phytol(I),dl-α-Tocopherol(II),5a-Ethoxy-α-tocopherol(Ⅲ) and 2,5-Cyclohexadiene-1,4-dione(Ⅳ).Compounds I-Ⅳ were isolated from D.indica for the first time.