Study on inhibitory action of luteolin-Cr(III) complex on α-glucosidase

Objective: To synthesize luteolin Cr(Ⅲ) complex and study the inhibitory action of luteolin and luteolin Cr(Ⅲ) on glucosi dase, determine the type of inhibitory action. Methods: The luteolin Cr(Ⅲ) complex was synthesized in dehydrated alcohol solution by mixing the luteolin and chromic acetate. After determining the structure of Luteolin Cr(Ⅲ) by IR、UV、TG DTA combined with element analysis method, the activity ofα glucosidase was investigated in the different pH and temperature. Inhibitory action of luteolin and luteolin Cr(Ⅲ) were tested onα glucosidase, and studied the kinetic characteristics by using Lineweaver Burk method. Results: The molecular composition of luteolin Cr(Ⅲ) was C15H9O6 Cr( COOCH3)2(H2O)2 ·2H2O. The optimum pH value ofα glucosidase was 510 temperature 55e. The IC50 value of inhibitory action of luteolin and luteolin Cr(Ⅲ) onα glucosidase were 1.496 mmol/ L and 0.2320 mmol/ L respectively. The type of inhibitory action was competitiveα glucosidase inhibitor. Conclusion: The luteolin Cr(Ⅲ) complex which was combined with luteolin and Cr3+was enhanced the inhibitory action onα glucosidase. It was a good foundation for further study of luteolin Cr(Ⅲ) complex.