Objective To investigate the effect of nonstrabismic anisometropia on binocular function. Methods Eighty-nine patients with hyperopic anisometropia were evaluated with the Worth Four-dot test, Bagolini lenses, a synoptophore, and Yan’s random dot stereogram. Fifty-six normal people were used as controls. Results With an increase in the amount of anisometropia, the incidence of distance suppression measured by both the Worth Four-dot test and stereoacuity increased. Most patients demonstrated fusion with Bagolini lenses. Most patients had distance stereopsis with a synoptophore and had a lower fusion amplitude than the control group (P0.05). Conclusion Anisometropia affects the development and maturation of binocularity, but could not completely destroy binocularity. Anisometropic people have weak fusion and coarse stereopsis.