To explore the methods of ensuring the successful laparoscopic operation, enhancing the quality of operation and prolonging the life-span of laparoscopes. Methods: Summarizing the application of 5S method in the administration of laparoscopes in the operation room since March of 1993. Results: All the laparoscopic operations were performed without any deficiency or shortfall of equipments. Conclusions: The application of 5S method is helpful to decrease the defects in laparoscopic operation nursing, to strengthen the initiative and sense of achievement among operation room nurses.