Tyrosinase detection via enzyme-triggered structural transformation of a ratiometric fluorescent probe

Tyrosinase is a key biomarker in melanoma, emphasizing the need for reliable detection. Here, we developed a ratiometric fluorescent probe that undergoes enzyme-triggered structural transformation, evidenced by HPLC and spectral changes. It enables sensitive analysis, inhibitor evaluation, and detection in spiked human serum, demonstrating potential for diagnostic applications.