Super-Resolution Imaging in Raman Microscopy

RamanResolution super- microscopyImaging super-resolution providesSuper resolution microscopic images of the sample with chemical information as the contrast. Since Raman microscopy uses light–material interactions associated withVibrational excitation vibrational excitationExcitation of molecules, the spatialResolution spatial resolution of the microscope is limited to the half of the wavelengthWavelength used to induce the Raman effect. In this chapter, we introduce various attempts for breaking the limit of the spatialResolution spatial resolution in Raman microscopy. Many of those techniques take similar approaches asResolution super- super-resolutionMicroscopy fluorescence fluorescence microscopyMicroscopy super-resolution , where the control of excitationExcitation and emission of fluorescence is the key to break the limit. Since there are many different types of Raman microscopy, such as spontaneousSpontaneous emission Raman scatteringRaman scattering spontaneous , coherent anti-Stokes Raman scattering, stimulated Raman scatteringRaman scattering stimulated , and so on, various approaches are proposed for spatio-temporalSpatiotemporal manipulation of the Raman effect in micro- and nanometerNanometer scale. In this chapter, we categorize the approaches to realizeSuper resolution super-resolutionResolution super- Raman imaging based on their strategies for breaking the limit and introduce the principlesPrinciples and the theoretical and experimental demonstrations of the techniques.