木犀科
基因沉默
树(集合论)
生物
病毒
基因
病毒学
植物
遗传学
数学
数学分析
作者
Κωνσταντίνος Κουδούνας,Margarita Thomopoulou,Elisavet Angeli,Dikran Tsitsekian,Stamatis Rigas,Polydefkis Hatzopoulos
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 165-182
被引量:8
标识
DOI:10.1007/978-1-0716-0751-0_13
摘要
Research on gene functions in non-model tree species is hampered by a number of difficulties such as time-consuming genetic transformation protocols and extended period for the production of healthy transformed offspring, among others. Virus-induced gene silencing (VIGS) is an alternative approach to transiently knock out an endogenous gene of interest (GOI) by the introduction of viral sequences encompassing a fragment of the GOI and to exploit the posttranscriptional gene silencing (PTGS) mechanism of the plant, thus triggering silencing of the GOI. Here we describe the successful application of Tobacco rattle virus (TRV)-mediated VIGS through agroinoculation of olive plantlets. This methodology is expected to serve as a fast tracking and powerful tool enabling researchers from diversified fields to perform functional genomic analyses in the olive tree.
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