[The Mechanism of Resveratrol on Acute T-Lymphocyte Leukemia through IL-7-Mediated JAK / STAT Signaling Pathway].

To explore the roles and relative mechanism of resveratrol against T-ALL through detecting the signaling molecules in IL-7 and JAK/STAT pathway.In vitro experiments, Molt4 cells were divided into 3 groups, including the control group, the DMSO group and resveratrol-treated group (Res group). The control group cells without any treatment, the DMSO group cells treated with 0.05% DMSO for 48 hours, the Res group cells treated with 200 μmol/L resveratrol for 48 hours. In vivo experiments, female C57BL/6J mice (6-8 weeks) were randomly divided into the control group, the T-ALL model group (T-ALL group), and Res treatment group (Res group). The control group mice treated with 0.05% DMSO by intragastric treatment, the T-ALL group mice treated with 0.05% DMSO by intragastric treatment, and the Res group mice treated with 10 mg/ml resveratrol. Expression of IL-7, IL-7R and Pim1 mRNA in the cells and mice spleen tissues were detected by RT-qPCR. Cell proliferation ability was detected by CCK-8. The expression of JAK1, JAK3, STAT5, phosphorylated JAK1 (p-JAK1), phosphorylated JAK3 (p-JAK3), phosphorylated STAT5 (p-STAT5) and Pim1 were detected by Western blot. ELISA was used to detect the IL-7 and IL-7R in the cells and mice serum of each groups.Resveratrol could inhibit the proliferation ability of Molt4 cells, decrease the relative levels of p-JAK1, p-JAK3, p-STAT5, Pim1 protein, and the expression levels of Pim1, IL-7 and IL-7R mRNA in cells and mice spleens, reduce the IL-7 and IL-7R in Molt4 cells and mice serum.Resveratrol may inhibite IL-7-medicated JAK/STAT signaling pathway to reduce the expression of target protein Pim1 to further exert its anti-T-ALL effects.IL-7介导的JAK/STAT信号通路在白藜芦醇抗急性T淋巴细胞白血病中的作用研究.观察T-ALL细胞株和小鼠模型中IL-7及JAK/STAT通路中信号分子的变化，探讨白藜芦醇抗T-ALL的作用机制.将T-ALL 细胞株Molt4分成对照、DMSO处理和白藜芦醇处理（Res）共3组，其中对照组不做任何处理，DMSO组用0.05% DMSO处理48 h，Res组用200 μmol/L白藜芦醇处理48 h。将15只6-8周龄健康雌性C57BL/6J小鼠随机分为正常对照、T-ALL模型（T-ALL）和白藜芦醇处理（Res）共3组，其中对照和T-ALL组均以 0.05% DMSO灌胃处理，Res组以10 mg/ml白藜芦醇灌胃处理。采用RT-qPCR法检测各组细胞和小鼠脾脏组织中IL-7、IL-7R及靶基因Pim1 mRNA的表达，CCK-8法检测细胞的增殖情况，Western blot检测各组细胞和小鼠脾脏组织JAK1、JAK3、STAT5、Pim1及磷酸化 JAK1（p-JAK1）、磷酸化 JAK3（p-JAK3）、磷酸化STAT5（p-STAT5）的蛋白表达，ELISA法检测各组细胞和小鼠血清中IL-7、IL-7R水平.Res抑制Molt4细胞增殖，下调Molt4细胞及T-ALL小鼠脾脏中p-JAK1、p-JAK3、p-STAT5、Pim1蛋白相对表达量以及Pim1、IL-7、IL-7R mRNA的表达水平，降低Molt4细胞及T-ALL小鼠血清中IL-7、IL-7R的水平（P<0.05）.白藜芦醇可能通过下调IL-7和IL-7R的表达水平，抑制其介导的JAK/STAT信号通路，进而降低靶蛋白Pim1表达发挥抗T-ALL的作用.