Protection against HPAI-H5N8 early challenge in commercial broilers with maternal immunity: prime-boost vs. single vaccination of a trivalent H5-inactivated vaccine

A phylogenetic study has proven vaccine genetic match with circulating H5N8 strains.Prime-boost vaccine regimen provided 100% survival in H5N8-challenged broilers.Enhanced immunity observed in prime-boost groups, with minimal viral shedding.Single-dose groups achieved 91.7% survival and showed higher viral shedding.