Enhanced humus synthesis from Chinese medicine residues composting by lignocellulose-degrading bacteria stimulation: Upregulation of key enzyme activity and neglected indirect effects on humus formation

Chinese medicine residues (CMHRs) resource is attracting widespread attention, as it is expected to be produced into Humus-rich fertilizer for soil application. This study aimed to promote effective humus (HS) production through lignocellulose-degrading bacteria (LDB) addition and explore the biological regulation mechanism of LDB affecting lignocellulose-to-humus conversion. The results showed higher HS production was achieved, with 109.73 and 111.44 g·kg-1, and HA/FA was raised by 12.70-16.02 % in compost products by LDB addition stimulation. Significant upregulation of β-glucanase and xylanase activities catalyzed higher decomposition of lignocellulose toward more HS potential precursors supply. Furthermore, exogenous LDB intervention induced microbial community restructure and microbial network establishment via enriching synergism functional bacteria, i.e., Thermobifida, Paenibacillus, Nonomuraea, etc. Mantel test results showed that it was variation of cellulose, hemicellulose and HS that affected microbial community succession (p < 0.01, r > 0.6), which represented the positive action of LDB addition stimulation on HS synthesis upregulation. Further exploration suggested LDB had an indirect effect on HS formation by enhanced lignin and hemicellulose conversion based on the Random Forest model and Partial least-squares path modeling results. This research provides new insights into the trigger effects of LDB introduction on upregulating HS synthesis and is expected to propose new perspectives for HS efficient production in CMHRs composting.