Abstract 2058: Expression of Brain MMP9 in a Rat Model of Cardiopulmonary Resuscitation Treated with SB-3CT

Over-expression of matrix metalloproteinase 9(MPP 9) in brain could degrade extracellular matrix, destroy the structure of microvascular basal membrane and vascular wall, take part in neural- inflammatory response in a rat model of cardiopulmonary resuscitation (CPR). SB-3CT is a antagonist of MPP 9. SB-3CT can inhibit over-expression of MPP 9 and reduce impairment of brain in a rat model of CPR. One hundred and twenty rats were randomly divided into 3 groups: the sham-operated group, the resuscitation control group and the resuscitation treatment group with SB-3CT. The rats were executed and the brains were taken out at 0h, 3h, 9h, 24h and 48h. The expression of MMP 9 in brain was detected. Water content and Evans blue content in brain tissue were observed. The expression of MMP 9 (Table 1 ), water content and Evans blue content in the resuscitation control group was obviously higher than the sham-operated group at 0h, 3h, 9h, 24h and 48h. The expression of MMP 9, water content and Evans blue content in the resuscitation treatment group was similar to the resuscitation control group at different time, but the level was lower than the resuscitation control group(p<0.05). SB-3CT as a specific antagonist of MMP9, could decrease the expression of MMP9 and decrease cerebral edema in a rat model of CPR, and it may be a useful traetment method for cerebral ischemia/reperfusion injury after CPR. Table 1 Expression of brain MMP 9 after resuscitation in a rat model of CPR