原位杂交
原位
寡核苷酸
信使核糖核酸
核糖核酸
生物
计算生物学
分子探针
分子生物学
内生
基因表达
杂交探针
低聚物限制
生物系统
化学
基因
生物化学
有机化学
作者
Arjun Raj,Sanjay Tyagi
出处
期刊:Methods in Enzymology
日期:2010-01-01
卷期号:: 365-386
被引量:169
标识
DOI:10.1016/s0076-6879(10)72004-8
摘要
Measurements of gene expression within single cells have revealed startling variability otherwise hidden in bulk measurements. Here, we present an in situ hybridization method capable of detecting individual mRNA molecules, thus permitting the accurate quantification and localization of mRNA within fixed sample. Our in situ protocol involves probing the target mRNA using a series of singly labeled oligonucleotide probes. This method is simple to implement and is applicable to a variety of biological samples. We also discuss some aspects of image processing required for analyzing the resulting data.
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