清脆的
简单
限制
背景(考古学)
功能(生物学)
困境
生物
过程(计算)
数据科学
风险分析(工程)
计算机科学
遗传学
工程类
基因
业务
操作系统
哲学
古生物学
认识论
机械工程
摘要
Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art instrumentation or the need for years of hands-on experience, for better or worse, ensures slow adoption by the community. CRISPR technology, however, presents the opposite dilemma, where the simplicity of the system enabled the parallel development of many applications, improvements and derivatives, and new users are now presented with an almost paralyzing abundance of choices. This Review intends to guide users through the process of applying CRISPR technology to their biological problems of interest, especially in the context of discovering gene function at scale.
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